Representative of temperance sent by Gloriana, the Fairy Queen, to destroy the Bower of Bliss, the garden belonging to the evil enchantress Acrasia, where many knights were lured to their lecherous dooms.

On the way, he was attacked, tempted, and robbed, but was victorious in each of his encounters. He was eventually assailed, while in a weakened condition, by two knights named Pyrochles and Cymochles, but he was rescued by Prince Arthur. Guyon and his mentor, Palmer, continued to the Bower of Bliss, where they were assaulted on all sides by sensual delights. Resisting them, they captured the sorceress Acrasia and destroyed the Bower.

Guyon had further adventures in Arthur’s company.